Burns : journal of the International Society for Burn Injuries · 2011
Effect of tetrandrine on the TGF-β-induced smad signal transduction pathway in human hypertrophic scar fibroblasts in vitro.
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Zunwen Lin, Shizhen Zhong, Dewu Liu, Yungui Mao, Pu Ning
Аннотация
PURPOSE: To evaluate in vitro effects of tetrandrine on the TGF-β-induced smad signal transduction pathway and cellular function altered by tetrandrine in human hypertrophic scar fibroblasts (HSFs).
METHODS: HSFs were exposed to four different treatments (control, tetrandrine, TGF-β(1) and mixture). After 48 h culture, expression of TGF-β1, Smad2 and Smad7 were examined using reverse transcription PCR and Western blotting. To evaluate cellular function alteration, morphological changes of HSFs were observed under an inverted microscope, expression of type I and III collagen were tested by immunocytochemistry, and cell cycles were analyzed by flow cytometry.
RESULTS: Expression of Smad7 increased while expression of Smad2 and TGF-β1 mRNA decreased in HSFs with tetrandrine. In addition, type I and III collagen was suppressed and S phase of the cell cycle markedly shortened in HSFs by tetrandrine.
CONCLUSIONS: These results suggest that tetrandrine inhibits HSFs at least partially through induction of Smad7 and decrement of Smad2 resulting in inhibition of TGF-β1 transcription and its intracellular signaling, which led to reduction of type I and III collagen production and suppression of cell reproductive activity.
METHODS: HSFs were exposed to four different treatments (control, tetrandrine, TGF-β(1) and mixture). After 48 h culture, expression of TGF-β1, Smad2 and Smad7 were examined using reverse transcription PCR and Western blotting. To evaluate cellular function alteration, morphological changes of HSFs were observed under an inverted microscope, expression of type I and III collagen were tested by immunocytochemistry, and cell cycles were analyzed by flow cytometry.
RESULTS: Expression of Smad7 increased while expression of Smad2 and TGF-β1 mRNA decreased in HSFs with tetrandrine. In addition, type I and III collagen was suppressed and S phase of the cell cycle markedly shortened in HSFs by tetrandrine.
CONCLUSIONS: These results suggest that tetrandrine inhibits HSFs at least partially through induction of Smad7 and decrement of Smad2 resulting in inhibition of TGF-β1 transcription and its intracellular signaling, which led to reduction of type I and III collagen production and suppression of cell reproductive activity.