[Detection of expression of heat shock protein 47 mRNA in pathological scar tissue by using real-time fluorescent quantitative RT-PCR].

OBJECTIVE

To detect the expression of heat shock protein 47 mRNA in pathological scar tissue by using real-time fluorescent quantitative reverse transcription-polymerase chain reaction (RT-PCR).

METHODS

The tissues of normal skin (n = 6), hypertrophic scar (n = 6) and keloid (n = 6) were adopted, which were diagnosed by Pathology Department. Based on fluorescent TaqMan methodology, the real-time fluorescent quantitative RT-PCR were adopted to detect the expression of heat shock protein 47 mRNA.

RESULTS

Compared with normal skin tissue (0.019 +/- 0.021) x 10(5), the expressions of heat shock protein 47 cDNA of hypertrophic scar tissue (1.233 +/- 1.039) x 10(5) and keloid tissue (1.222 +/- 0.707) x 10(5) were higher, being significant differences (P < 0.05).

CONCLUSION

A fluorescent quantitative method was successfully applied to detecting the expression of heat shock protein 47 mRNA. Heat shock protein 47 may play an important role in promoting the formation of pathological scar tissue.