Odor Perception in the Cotton Bollworm, Helicoverpa armigera, Exposed to Juglans regia, a Marginal Host Plant.

The cotton bollworm, Helicoverpa armigera, is one of the most destructive agricultural pests in the world, infesting cotton, maize, soybean, and many other crops. In recent years, H. armigera has been observed damaging walnuts, Juglans regia, in Xinjiang China. Here we examine the chemical perception by H. armigera of the marginal host J. regia. In Y-tube olfactometer tests, we found H. armigera females and males both showed significant behavioral responses to odors from walnut branches. Furthermore, nine electrophysiologically active volatiles (α-pinene, β-pinene, myrcene, limonene, eucalyptol, ocimene, β-caryophyllene, (E)-β-farnesene, and germacrene D) were identified from walnuts with gas chromatography coupled with electroantennography (GC-EAD) and gas chromatography-mass spectrometry (GC-MS). Among these volatiles, β-pinene and eucalyptol were released in relatively higher amounts. In electroantennogram (EAG) dose-dependent trials, all compounds evoked responses in H. armigera adults when tested at high concentrations, with germacrene D evoking the greatest response. In wind tunnel tests, H. armigera females preferred eight of the electrophysiologically active volatile dilutions compared with clean air, while males showed preference for only five compounds. As such we describe the chemical recognition of H. armigera for walnut, a marginal host. This study contributes to understanding the interaction between polyphagous pests and their host plants.