Three new single nucleotide polymorphisms identified in Plasmodium falciparum isolates from Kogi State, Nigeria.

BACKGROUND

High malaria burden remains a remarkable challenge in Nigeria. The high prevalence has been attributed to a number of factors, including the continued evolution of resistance to antimalarial drugs by Plasmodium. However, the actual occurrence of antimalarial drug resistance in the country has not been well-documented. This study was aimed at screening for molecular markers of multidrug resistant Plasmodium species in individuals with parasitaemia in Kogi State, Nigeria.

METHOD: Blood samples drawn from 600 symptomatic patients who consented to be recruited for the study were tested by Dipstick antigen test (RDT) for malaria. Genomic DNA from 40 samples, confirmed positive for P. falciparum by the rapid diagnostic test and PCR, were screened for the Pfmdr1 and Kelch-13 genes. Some of the Kelch-13 PCR amplicons were randomly selected and sequenced using Sanger method. Thereafter, the sequences were analysed and compared with wild type for single nucleotide polymorphisms (SNPs).

RESULTS

Pfmdr1 and K-13 amplicons were successfully obtained from 45 % (18/40) of the malaria-positive blood samples. Bioinformatics analysis of the K-13 sequences showed near 100 % sequence homology and percentage similarity of 99.88 % with the wild type Plasmodium falciparum 3D7 (Accession Number XM_001350122) gene. There were however three non-synonymous mutations (K430E, W706C and Q707K) observed in three samples.

CONCLUSIONS

SNP analysis showed none of these mutations has been previously associated with artemisinin resistance. The significance of the study results was discussed in relation to current national malaria elimination and antimalarial resistance strategic plan or policy.