Characterization of sand fly (Diptera: Psychodidae: Phlebotominae) fauna and potential hosts of Leishmania in a military area of Dourados, Mato Grosso do Sul, Brazil.

We investigated the sand fly fauna and the presence of Leishmania spp. DNA in collected specimens, domestic animals, and military personnel. The 4th Mechanized Cavalry Brigade of Dourados, Mato Grosso do Sul, Brazil is located in an area that has characteristics conducive to the life cycle of Leishmania spp. An epidemiological questionnaire applied to military personnel, and whole blood samples were collected from them and domestic animals. Using the collected blood samples, PCR (Polymerase Chain Reaction) and serology tests were performed to detect Leishmania spp. DNA and anti-Leishmania antibodies, respectively. Additionally, CDC (Center on Disease Control) traps were installed at eight points over 12 months. In total, 652 sand flies belonging to 9 genera and 19 species were collected. Lutzomyia longipalpis, which is the main vector of Leishmania infantum, the etiological agent of visceral leishmaniasis was present in half of the collection points. The DNA detection rate of Leishmania spp. was 0.31% (one pool of Psathyromyia aragaoi and one pool of Brumptomyia spp.). We analyzed 35 animals (dogs and horses) and 235 military personnel. Analysis of samples from animals and soldiers was negative for Leishmania spp. About 30.2% (n = 71) of military personnel did not know about leishmaniasis. Although some vector species were found, no Leishmania spp. DNA was identified in species of medical and veterinary importance. The area investigated showed characteristics that can contribute to the proliferation of the vector and the spread of the disease. Long-term monitoring studies and activities that increase awareness in the population should be encouraged by research funding bodies and leishmaniasis surveillance and control programs.