[Effects of asiaticoside on cell proliferation and Smad signal pathway of hypertrophic scar fibroblasts].

OBJECTIVE

To investigate the effects of asiaticoside on the proliferation and the Smad signal pathway of the hypertrophic scar fibroblasts.

METHODS

The hypertrophic scar fibroblasts were cultured with tissue culture method. The expressions of Smad2 and Smad7 mRNA after asiaticoside treatment were determined by reverse transcription-polymerase chain reaction 48 hours later. The cell cycle, the cell proliferation, the cell apoptosis and the expression of phosphorylated Smad2 and Smad7 with (experimental group) or without (control group) asiaticoside were detected with flow cytometry, immunocytochemistry and Western blot.

RESULTS

Asiaticoside inhibited the hypertrophic scar fibroblasts from phase S to phase M. The Smad7 content and the expression of Smad7 mRNA were (1.33+/-1.26)% and (50.80+/-22.40)% in experimental group, and (9.15+/-3.36)% and (32.18+/-17.84)% in control group; there were significant differences between two groups (P<0.05). While the content and the mRNA expression of Smad2 had no significant difference between two groups.

CONCLUSION

Asiaticoside inhibits the scar formation through Smad signal pathway.