[Gene expression of stress activated protein kinase and its MAPKs in hypertrophic scar].

OBJECTIVE

To explore the change of gene expression of stress activated protein kinase (SAPK) and its upstream signal-regulated molecule--mitogen activated protein kinases(MAPKs) (MKK4 and MKK7) in hypertrophic scar and auto-control normal skin.

METHODS

The total RNA was isolated from 8 hypertrophic scars and 8 auto-control skin, and then mRNA was purified. The gene expressions of MKK4, MKK7 and SAPK were examined with reverse transcription-polymerase chain reaction(RT-PCR) method.

RESULTS

In hypertrophic scar, both MKK7 and SAPK genes weakly expressed. In auto-control skin, the expression of these 2 genes was significantly elevated in comparison with hypertrophic scar (P < 0.01). The expression levels of these 2 genes were 1.5 times and 2.6 times as long as those of hypertrophic scar, respectively. Gene expression of MKK4 had no significant difference between auto-control skin and hypertrophic scar (P > 0.05).

CONCLUSION

Decreased gene expression of MKK7 and SAPK which results in reducing cell apoptosis might be one of the mechanisms for controlling the formation of hypertrophic scar.