Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue · 2003
[Gene expression of extracellular-signal regulated protein kinase 1/2 and their MAPKKs in hypertrophic scars].
Аннотация на английскомСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Chen Wei, Fu Xiao-bing, Sun Xiao-qing, Sun Tong-zhu, Zhao Zhi-li, Zhou Gang, Yan Yin-hui, Sheng Zhi-yong
Аннотация
OBJECTIVE: To explore the change of extracellular-signal regulated protein kinase 1/2 (ERK1/2) and their mitogen activated protein kinase kinases(MAPKKs) gene expression in normal skins versus hypertrophic scars underlying their biological significances.
METHODS: After total RNAs were isolated from 8 specimens of hypertrophic scars and 8 specimens of normal skins, and then were purified to mRNAs, the gene expression of ERK1/2 and MEK1/2 were examined with reverse transcription-polymerase chain reaction (RT-PCR).
RESULTS: In normal skins, the intensity of gene expression of ERK1/2 and MEK1/2 were weak. In hypertrophic scar, the expression intensities of these 4 genes were 1.1 times, 1.2 times, 2.2 times and 2.5 times of normal skins, respectively. The expression of these 4 genes was significantly elevated in comparison with normal skins.
CONCLUSION: The activation of MEK1/2, ERK1/2 gene expression in hypertrophic scars versus in normal skins might be one of the mechanisms controlling the formation of hypertrophic scars.
METHODS: After total RNAs were isolated from 8 specimens of hypertrophic scars and 8 specimens of normal skins, and then were purified to mRNAs, the gene expression of ERK1/2 and MEK1/2 were examined with reverse transcription-polymerase chain reaction (RT-PCR).
RESULTS: In normal skins, the intensity of gene expression of ERK1/2 and MEK1/2 were weak. In hypertrophic scar, the expression intensities of these 4 genes were 1.1 times, 1.2 times, 2.2 times and 2.5 times of normal skins, respectively. The expression of these 4 genes was significantly elevated in comparison with normal skins.
CONCLUSION: The activation of MEK1/2, ERK1/2 gene expression in hypertrophic scars versus in normal skins might be one of the mechanisms controlling the formation of hypertrophic scars.