Zhonghua zheng xing wai ke za zhi = Zhonghua zhengxing waike zazhi = Chinese journal of plastic surgery · 2001
[The biological effect of verapamil on hypertrophic scar fibroblast].
Аннотация на английскомСтатьяНазвание и аннотация: Перевод пока не подготовлен
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Wang Z, Guo S, Lu K
Аннотация
OBJECTIVE: To study the mechanism of calcium channel antagonist Verapamil on the treatment of hypertrophic scar and explore the possibility of further clinical application.
METHODS: After six strains of HSFB were cultured in vitro, we investigated HSFB proliferation by MTT method, investigated HSFB collagen synthesis by 3H-proline uptaken and Hydroxyproline colorimetric analysis, and investigated collagen gene expression by Northern Blot.
RESULTS: Verapamil can inhibit HSFB proliferation, collagen synthesis and gene expression by a dose-depended manner, especially treated with 100 mumol/L Verapamil.
CONCLUSION: By inhibiting I, III procollagen gene expression, Verapamil can inhibit the formation of hypertrophic scar.
METHODS: After six strains of HSFB were cultured in vitro, we investigated HSFB proliferation by MTT method, investigated HSFB collagen synthesis by 3H-proline uptaken and Hydroxyproline colorimetric analysis, and investigated collagen gene expression by Northern Blot.
RESULTS: Verapamil can inhibit HSFB proliferation, collagen synthesis and gene expression by a dose-depended manner, especially treated with 100 mumol/L Verapamil.
CONCLUSION: By inhibiting I, III procollagen gene expression, Verapamil can inhibit the formation of hypertrophic scar.