[The biological effect of verapamil on hypertrophic scar fibroblast].

OBJECTIVE

To study the mechanism of calcium channel antagonist Verapamil on the treatment of hypertrophic scar and explore the possibility of further clinical application.

METHODS

After six strains of HSFB were cultured in vitro, we investigated HSFB proliferation by MTT method, investigated HSFB collagen synthesis by 3H-proline uptaken and Hydroxyproline colorimetric analysis, and investigated collagen gene expression by Northern Blot.

RESULTS

Verapamil can inhibit HSFB proliferation, collagen synthesis and gene expression by a dose-depended manner, especially treated with 100 mumol/L Verapamil.

CONCLUSION

By inhibiting I, III procollagen gene expression, Verapamil can inhibit the formation of hypertrophic scar.