[Experimental study of Bcl-2 and Fas gene expression in fibroblast of scar].

OBJECTIVE

To explore the effect of Fas/Apo-1 and Bcl-2 gene expression on mechanism of scar formation.

METHODS

Immunohistochemical method was applied to defect the expression of Fas and Bcl-2 protein in fibroblasts from 10 cases with normal skin, 10 cases with hypertrophic scar and 10 cases with keloid.

RESULTS

The positive expression rate of Bcl-2 protein in keloid was 83.2%, significantly higher than that in hypertrophic scar (38.6%), (P < 0.01), and the positive expression rate in hypertrophic scar and keloid was higher than that in normal skin (6.78%), (P < 0.01). But the positive expression rate of Fas/Apo-1 protein was 78.4% in normal skin 80.4% in hypertrophic scar, 84.4% in keloid respectively, which showed no significant difference among them (P > 0.05).

CONCLUSION

Bcl-2 gene but Fas gene may take part in the formation of pathologic scar.