Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control · 2012
[Prediction and identification of Taenia solium oncosphere TSO45-4B antigen FnIII structure domain linear B cell epitopes].
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Wang Yuan-Yuan, Wang Xiao-Li, Chang Xue-Lian, Tao Zhi-Yong, Xia Hui, Fang Qiang
Аннотация
OBJECTIVE: To predict and identify Taenia solium oncosphere TSO45-4B antigen Fn III structure domain linear B cell epitopes.
METHODS: The B cell epitopes of TSO45-4B Fn III structure domain were predicted through the sequence analysis by using bioinformatics online tools and the protein space conformation was predicted by SWISS-MODEL. The peptides were synthesized according to the predicted linear epitopes. The immunoreactivity of sera of cysticercosis patients to the peptides synthesized was tested by using ELISA.
RESULTS: Two linear B cell epitopes of TSO45-4B Fn III structure domain were predicted, and one of the predicted epitope peptides synthesized could be recognized by the sera of cysticercosis patients.
CONCLUSION: Two linear B cell epitopes of TSO45-4B Fn III structure domain are predicted and one of them has been confirmed successfully.
METHODS: The B cell epitopes of TSO45-4B Fn III structure domain were predicted through the sequence analysis by using bioinformatics online tools and the protein space conformation was predicted by SWISS-MODEL. The peptides were synthesized according to the predicted linear epitopes. The immunoreactivity of sera of cysticercosis patients to the peptides synthesized was tested by using ELISA.
RESULTS: Two linear B cell epitopes of TSO45-4B Fn III structure domain were predicted, and one of the predicted epitope peptides synthesized could be recognized by the sera of cysticercosis patients.
CONCLUSION: Two linear B cell epitopes of TSO45-4B Fn III structure domain are predicted and one of them has been confirmed successfully.