Journal of global antimicrobial resistance · 2021
Draft genome sequence of a lipopeptide-producing Bacillus amyloliquefaciens strain isolated from wheat field soil with antagonistic activity against azole-resistant Fusarium graminearum.
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Wang Yuting, Xu Jianhong, Shi Jianrong, Ma Guizhen, Wang Gang
Аннотация
OBJECTIVES: Lipopeptides have been revealed as good potential biocontrol agents against various pathogenic microbes. In the present work, we report the draft genome sequence of a lipopeptide-producing strain of Bacillus amyloliquefaciens (7D3) that showed good antifungal activity against the azole-resistant pathogenic fungus Fusarium graminearum.
METHODS: Whole-genome sequencing of strain 7D3 was performed on an Illumina MiSeq 300 platform. Raw data were cleaned using Trim Galore v.0.4.0 and were checked for quality using FastQC. De novo assembly was performed using the SOAPdenovo2 package. Genes responsible for the biosynthesis of secondary metabolites were identified using antiSMASH.
RESULTS: Bacillus amyloliquefaciens 7D3 genome assembly resulted in a total genome size of 3 913 220 bp with a G+C content of 46.13%. There were 3998 predicted genes with 72 tRNAs and 9 rRNAs. A total of ten gene clusters were found to be related to secondary metabolite biosynthesis, of which five were identified as lipopeptide synthesis clusters.
CONCLUSION: This study presents the genome sequence of B. amyloliquefaciens 7D3, which exhibited intense antagonistic activity against azole-resistant fungi. The whole genome sequence will help in the search for novel antifungal peptides against drug-resistant pathogens.
METHODS: Whole-genome sequencing of strain 7D3 was performed on an Illumina MiSeq 300 platform. Raw data were cleaned using Trim Galore v.0.4.0 and were checked for quality using FastQC. De novo assembly was performed using the SOAPdenovo2 package. Genes responsible for the biosynthesis of secondary metabolites were identified using antiSMASH.
RESULTS: Bacillus amyloliquefaciens 7D3 genome assembly resulted in a total genome size of 3 913 220 bp with a G+C content of 46.13%. There were 3998 predicted genes with 72 tRNAs and 9 rRNAs. A total of ten gene clusters were found to be related to secondary metabolite biosynthesis, of which five were identified as lipopeptide synthesis clusters.
CONCLUSION: This study presents the genome sequence of B. amyloliquefaciens 7D3, which exhibited intense antagonistic activity against azole-resistant fungi. The whole genome sequence will help in the search for novel antifungal peptides against drug-resistant pathogens.