Investigative ophthalmology & visual science · 2020
Endothelial Yes-Associated Protein 1 Promotes Astrocyte Proliferation and Maturation via Cytoplasmic Leukemia Inhibitory Factor Secretion in Oxygen-Induced Retinopathy.
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Ai Li-Qian-Yu, Zhu Jing-Yi, Chen Xi, Li Xue, Luo Lin-Lin, Hu Qiu-Mei, Lin Sen, Ye Jian
Аннотация
PURPOSE: Purpose The role of endothelial Yes-associated protein 1 (YAP) in the pathogenesis of retinal angiogenesis and the astrocyte network in the mouse oxygen-induced retinopathy (OIR) model is unknown.
METHODS: For in vivo studies, OIR was induced in conditional endothelial YAP knockout mice and their wild-type littermates. Retinal vascularization and the astrocyte network were evaluated by whole-mount fluorescence and Western blotting. In vitro experiments were performed in astrocytes cultured with human microvascular endothelial cell-1-conditioned medium to analyze the mechanisms underlying the effect of endothelial YAP on astrocytes.
METHODS: For in vivo studies, OIR was induced in conditional endothelial YAP knockout mice and their wild-type littermates. Retinal vascularization and the astrocyte network were evaluated by whole-mount fluorescence and Western blotting. In vitro experiments were performed in astrocytes cultured with human microvascular endothelial cell-1-conditioned medium to analyze the mechanisms underlying the effect of endothelial YAP on astrocytes.
Предметные рубрики MeSH
Adaptor Proteins, Signal TransducingAnimalsAnimals, NewbornAstrocytesBlotting, WesternCell Cycle ProteinsCell ProliferationCells, CulturedCulture Media, ConditionedCytoplasmDisease Models, AnimalEndothelial CellsFluorescent Antibody Technique, IndirectLeukemia Inhibitory FactorMiceMice, Inbred C57BLMice, KnockoutMice, TransgenicOxygenRetinal NeovascularizationRetinal VesselsRetinopathy of PrematurityYAP-Signaling Proteins