Risk of exposure to bioaerosols in clinical environments: Bronchoscopy.

BACKGROUND

Health care professionals face a heightened risk of occupational infections, with procedures such as bronchoscopy further increasing this risk by generating aerosols containing diverse microorganisms. This study quantified bioaerosol concentrations during bronchoscopy to evaluate occupational exposure.

METHODS

Aerosol samples were collected during routine bronchoscopies in 2 hospitals using personal samplers with quartz filters attached to health care professional's clothing. Samples were cultured on various media, with bacterial colonies identified by 16S rDNA sequencing and fungal colonies by ITS1-2 region sequencing. Respiratory viruses (SARS-CoV-2, influenza A/B, RSV) were detected via RT-PCR.

RESULTS

A total of 44 aerosol samples were collected: 7 pre-procedure, 32 during bronchoscopy, and 5 between patients. From samples taken during bronchoscopies, 287 microbial colonies were isolated. The most abundant bacterial genera were Micrococcus, Staphylococcus, and Bacillus, while fungal genera included Aspergillus, Talaromyces and Cladosporium. Several potentially pathogenic species were identified, such as Staphylococcus aureus, Kocuria rosea, and Kroppenstedtia pulmonis, as well as some pathogenic fungi. A total of 8 previously unsuspected SARS-CoV-2 positive samples were also detected. Our estimates suggest that an interventionalist, without protective equipment, could be exposed to approximately 98.94 CFU/m3 during a typical procedure.

CONCLUSIONS

Bronchoscopy generates aerosols containing microorganisms from the oral, nasal, and pulmonary microbiota, as well as potential pathogens. These aerosols can be inhaled by health care workers, representing a risk of occupational infection.