BMC immunology · 2026
MiR-100-5p is involved in severe pneumonia in children by targeting MTOR.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Zhang Lun, Wang Qing, Mo Shoushan
Аннотация
BACKGROUND: Severe pneumonia in children is a life-threatening respiratory condition that can lead to severe complications and even mortality. We confirmed the possibility of miR-100-5p as a biomarker in severe pneumonia in children.
METHODS: A total of 200 children were included. The expression of miR-100-5p from serum was detected. WI-38 cells were treated with 10 µg/mL LPS for 24 h. miR-100-5p was overexpressed or inhibited by transfection. In addition, proliferation and inflammatory factors were measured. TargetScan was utilized to forecast the downstream of miR-100-5p. The targeting relationship between miR-100-5p and MTOR was confirmed by the dual-luciferase reporter system. miR-100-5p and MTOR were co-overexpressed to detect the function of both in severe pneumonia in children.
RESULTS: miR-100-5p expression was decreased and had a high diagnostic value in severe pneumonia in children. The miR-100-5p level was closely related to clinical indicators. In WI-38 cells treated with LPS, overexpression of miR-100-5p increased proliferation and reduced inflammatory factors, while inhibition of miR-100-5p reduced proliferation and increased inflammatory factors. MTOR expression was negatively associated with miR-100-5p expression in severe pneumonia in children.
CONCLUSIONS: miR-100-5p may participate in severe pneumonia in children by negatively regulating MTOR, proving that it might be a biomarker of severe pneumonia in children.
METHODS: A total of 200 children were included. The expression of miR-100-5p from serum was detected. WI-38 cells were treated with 10 µg/mL LPS for 24 h. miR-100-5p was overexpressed or inhibited by transfection. In addition, proliferation and inflammatory factors were measured. TargetScan was utilized to forecast the downstream of miR-100-5p. The targeting relationship between miR-100-5p and MTOR was confirmed by the dual-luciferase reporter system. miR-100-5p and MTOR were co-overexpressed to detect the function of both in severe pneumonia in children.
RESULTS: miR-100-5p expression was decreased and had a high diagnostic value in severe pneumonia in children. The miR-100-5p level was closely related to clinical indicators. In WI-38 cells treated with LPS, overexpression of miR-100-5p increased proliferation and reduced inflammatory factors, while inhibition of miR-100-5p reduced proliferation and increased inflammatory factors. MTOR expression was negatively associated with miR-100-5p expression in severe pneumonia in children.
CONCLUSIONS: miR-100-5p may participate in severe pneumonia in children by negatively regulating MTOR, proving that it might be a biomarker of severe pneumonia in children.