Annals of clinical and translational neurology · 2025
High-Throughput Immunoassays for Cavin-4 IgG: A Diagnostic Tool for Immune-Mediated Rippling Muscle Disease.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
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LaFrance-Corey Reghann G, Kherbek Haidara, Harinesan Nimalan, Milone Margherita, Paramasivan Naveen K, Sarker Pallab, Knight Andrew M, Karsten Carley, Dasari Surendra, Liewluck Teerin, Litchy William J, Pittock Sean J, Mills John R, Dubey Divyanshu
Аннотация
Cavin-4 was identified as a potential autoantigen for immune-mediated rippling muscle disease (iRMD). To validate this, we developed and tested various immunoassays, including a cell-based assay (CBA), cavin-4 recombinant protein ELISA, and multi-peptide ELISA. Among 19 iRMD patients, all exhibited muscle rippling, and 13 had percussion-induced mounding. All immunoassays demonstrated clinical and analytical specificities greater than 95%. The protein ELISA had the highest sensitivity (94.7%) and specificity (99.9%), outperforming CBA (sensitivity 89.5%, specificity 99.6%) and the multi-peptide ELISA (sensitivity 79.0%, specificity 97.2%). Our results suggest that the cavin-4 protein ELISA is a promising tool for high-throughput clinical testing in iRMD.