Studies on the appearance of chalone proteins in subcellular fractions from some bovine tissues. P. II Attempt of isolation of intracellular glycoproteins (chalone) from bovine spleen.

In the course of purification of chalone deriving from the subcellular fractions of bovine spleens, the butanol extraction of smooth and rough microsomes supernatants was performed. After extraction the lower layer was treated with SDS, buffered at pH 5,8. The homogeneity of obtained fractions was investigated by means of polyacrylamide electrophoresis. The antimitotic activity of fractions was performed by colcemide test in mice. There was stated that the cells of spleen contained two chalones. One of them showed antimitotic activity towards spleens of mice, the other towards lymph nodes. The former revealed on the polyacrylamide gel the mobility of gamma or beta, globulins, the later of prealbumins.