Molecular genetics and metabolism · 2013
Rapid assays for Gaucher and Hurler diseases in dried blood spots using digital microfluidics.
СтатьяПоддержка NIH: внешняя программаНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Sista Ramakrishna S, Wang Tong, Wu Ning, Graham Carrie, Eckhardt Allen, Bali Deeksha, Millington David S, Pamula Vamsee K
Аннотация
OBJECTIVE: Easy tool for newborn screening of Gaucher and Hurler diseases.
METHODS: Method comparison between fluorometric enzymatic activity assay on a digital microfluidic platform and micro-titer plate bench assay was performed on normal (n = 100), Gaucher (n = 10) and Hurler (n = 7) dried blood spot samples.
RESULTS: Enzymatic activity analysis of glucocerebrosidase (Gaucher) and α-l-iduronidase (Hurler) revealed similar discrimination between normal and affected samples on both platforms.
CONCLUSIONS: Digital microfluidics is suitable for Gaucher and Hurler newborn screening.
METHODS: Method comparison between fluorometric enzymatic activity assay on a digital microfluidic platform and micro-titer plate bench assay was performed on normal (n = 100), Gaucher (n = 10) and Hurler (n = 7) dried blood spot samples.
RESULTS: Enzymatic activity analysis of glucocerebrosidase (Gaucher) and α-l-iduronidase (Hurler) revealed similar discrimination between normal and affected samples on both platforms.
CONCLUSIONS: Digital microfluidics is suitable for Gaucher and Hurler newborn screening.