Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology · 2009
[Genetic analysis of a strain of adenovirus Ad7d2 isolated from an infant died of pneumonia].
Клинический случайАннотация на английскомСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Tang Liu-ying, Liu Xiu-yun, Xu Wen-bo
Аннотация
OBJECTIVE: To analyze the genetic characteristics of a strain of adenovirus, Ad7d2, isolated from an infant died of severe pneumonia.
METHODS: Virus isolation was performed by using the nasopharyngeal secretion from an 11-month-old infant with serious pneumonia. The viral DNA was amplified by PCR and the products were sequenced.
RESULTS: One strain of virus was isolated and was named as BJ060316-1. Sequence analysis of the hexon and fiber gene of the PCR products showed that the strain was Ad7d2, which shared 99.5% homology for 950bp hexon fragment with AF321311 Ad7d2 isolated from Israel in 1993. Blast with deduced amino acid sequence showed that BJ060316-1 lost glutamine at site 253, and at the site 495 arginine replaced serine. For fiber gene, BJ060316-1 showed 99.7% homology with AB243118 Ad7 isolated in Japan in 2005 for 975 bp fragment.
CONCLUSION: Adenovirus Ad7d2 strain BJ060316-1 isolated from a an infant with fatal pneumonia showed no virulence mutation.
METHODS: Virus isolation was performed by using the nasopharyngeal secretion from an 11-month-old infant with serious pneumonia. The viral DNA was amplified by PCR and the products were sequenced.
RESULTS: One strain of virus was isolated and was named as BJ060316-1. Sequence analysis of the hexon and fiber gene of the PCR products showed that the strain was Ad7d2, which shared 99.5% homology for 950bp hexon fragment with AF321311 Ad7d2 isolated from Israel in 1993. Blast with deduced amino acid sequence showed that BJ060316-1 lost glutamine at site 253, and at the site 495 arginine replaced serine. For fiber gene, BJ060316-1 showed 99.7% homology with AB243118 Ad7 isolated in Japan in 2005 for 975 bp fragment.
CONCLUSION: Adenovirus Ad7d2 strain BJ060316-1 isolated from a an infant with fatal pneumonia showed no virulence mutation.