Clinical and experimental dermatology · 2018
Potassium hydroxide mount with cellophane adhesive tape: a method for direct diagnosis of dermatophyte skin infections.
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Raghukumar S, Ravikumar B C
Аннотация
BACKGROUND: Potassium hydroxide (KOH) mount is a simple and rapid procedure that can be performed with minimal resources and provides results within 1-2 h.
AIM: To further optimize KOH mount using transparent cellophane tape to ascertain fungal elements, even by inexperienced personnel.
METHODS: A split-body study was conducted on 100 patients with tinea cruris. Conventional scrape method was performed on the right side and the cellophane tape method on the left. Microscopy was performed 1-2 h after collection, and on the next morning and days 3 and 7.
RESULTS: Of the 100 samples, positive results were seen in 91 (91%) with the standard method and 94 (94%) with the cellophane tape method. Clumping of the fungi and presence of artefacts were more marked with the scrape than with the tape method.
CONCLUSION: In view of its merits over the conventional scrape method, the cellophane tape method is a prudent alternative for the detection of superficial fungal infections.
AIM: To further optimize KOH mount using transparent cellophane tape to ascertain fungal elements, even by inexperienced personnel.
METHODS: A split-body study was conducted on 100 patients with tinea cruris. Conventional scrape method was performed on the right side and the cellophane tape method on the left. Microscopy was performed 1-2 h after collection, and on the next morning and days 3 and 7.
RESULTS: Of the 100 samples, positive results were seen in 91 (91%) with the standard method and 94 (94%) with the cellophane tape method. Clumping of the fungi and presence of artefacts were more marked with the scrape than with the tape method.
CONCLUSION: In view of its merits over the conventional scrape method, the cellophane tape method is a prudent alternative for the detection of superficial fungal infections.