Scientific reports · 2016
Rapid diagnosis of lymph node metastasis in breast cancer using a new fluorescent method with γ-glutamyl hydroxymethyl rhodamine green.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Shinden Yoshiaki, Ueo Hiroki, Tobo Taro, Gamachi Ayako, Utou Mitsuaki, Komatsu Hisateru, Nambara Sho, Saito Tomoko, Ueda Masami, Hirata Hidenari, Sakimura Shotaro, Takano Yuki, Uchi Ryutaro, Kurashige Junji, Akiyoshi Sayuri, Iguchi Tomohiro, Eguchi Hidetoshi, Sugimachi Keishi, Kubota Yoko, Kai Yuichiro, Shibuta Kenji, Kijima Yuko, Yoshinaka Heiji, Natsugoe Shoji, Mori Masaki, Maehara Yoshihiko, Sakabe Masayo, Kamiya Mako, Kakareka John W, Pohida Thomas J, Choyke Peter L, Kobayashi Hisataka, Ueo Hiroaki, Urano Yasuteru, Mimori Koshi
Аннотация
Sentinel lymph node biopsy is performed as a standard procedure in breast cancer surgery, and the development of quick and simple methods to detect metastatic lesions is in high demand. Here, we validated a new fluorescent method using γ-glutamyl hydroxymethyl rhodamine green to diagnose metastatic lymph nodes in breast cancer. One hundred and forty-nine lymph nodes from 38 breast cancer patients were evaluated in this study. Comparison of fluorescent and pathological images showed that this fluorescent method was successful for visualizing breast cancer cells in lymph nodes. This method had a sufficiently high sensitivity (97%), specificity (79%) and negative predictive value (99%) to render it useful for an intraoperative diagnosis of cancer. These preliminary findings suggest that this novel method is useful for distinguishing non-cancerous specimens from those in need of careful examination and could help save time and cost for surgeons and pathologists.