Journal of neural engineering · 2020
First steps for the development of silk fibroin-based 3D biohybrid retina for age-related macular degeneration (AMD).
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Jemni-Damer Nahla, Guedan-Duran Atocha, Cichy Jasmin, Lozano-Picazo Paloma, Gonzalez-Nieto Daniel, Perez-Rigueiro José, Rojo Francisco, V Guinea Gustavo, Virtuoso Assunta, Cirillo Giovanni, Papa Michele, Armada-Maresca Félix, Largo-Aramburu Carlota, Aznar-Cervantes Salvador D, Cenis José L, Panetsos Fivos
Аннотация
UNLABELLED: Age-related macular degeneration is an incurable chronic neurodegenerative disease, causing progressive loss of the central vision and even blindness. Up-to-date therapeutic approaches can only slow down he progression of the disease.
OBJECTIVE: Feasibility study for a multilayered, silk fibroin-based, 3D biohybrid retina.
APPROACH: Fabrication of silk fibroin-based biofilms; culture of different types of cells: retinal pigment epithelium, retinal neurons, Müller and mesenchymal stem cells ; creation of a layered structure glued with silk fibroin hydrogel.
MAIN RESULTS: In vitro evidence for the feasibility of layered 3D biohybrid retinas; primary culture neurons grow and develop neurites on silk fibroin biofilms, either alone or in presence of other cells cultivated on the same biomaterial; cell organization and cellular phenotypes are maintained in vitro for the seven days of the experiment.
SIGNIFICANCE: 3D biohybrid retina can be built using silk silkworm fibroin films and hydrogels to be used in cell replacement therapy for AMD and similar retinal neurodegenerative diseases.
OBJECTIVE: Feasibility study for a multilayered, silk fibroin-based, 3D biohybrid retina.
APPROACH: Fabrication of silk fibroin-based biofilms; culture of different types of cells: retinal pigment epithelium, retinal neurons, Müller and mesenchymal stem cells ; creation of a layered structure glued with silk fibroin hydrogel.
MAIN RESULTS: In vitro evidence for the feasibility of layered 3D biohybrid retinas; primary culture neurons grow and develop neurites on silk fibroin biofilms, either alone or in presence of other cells cultivated on the same biomaterial; cell organization and cellular phenotypes are maintained in vitro for the seven days of the experiment.
SIGNIFICANCE: 3D biohybrid retina can be built using silk silkworm fibroin films and hydrogels to be used in cell replacement therapy for AMD and similar retinal neurodegenerative diseases.