Leukemia research · 2010
Up-regulation of ABCB1/P-glycoprotein by escaping promoter hypermethylation indicates poor prognosis in hematologic malignancy patients with and without bone marrow transplantation.
СтатьяПоддержка исследования: вне правительства СШАПоддержка правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Shi Cheng-Jun, Wang Fang, Ren Mei-Feng, Mi Yan-Jun, Yan Yan-Yan, To Kenneth Kin Wah, Dai Chun-Ling, Wang Yan-Sheng, Chen Li-Ming, Tong Xiu-Zhen, Liang Yong-Ju, Fu Li-Wu
Аннотация
We investigated the correlation between MDR1 promoter methylation status and MDR1 expression in 228 hematologic malignancies patients and 90 healthy controls. High level of MDR1 mRNA correlated to promoter hypomethylation and strongly associated with poor prognosis indicated by 2-year survival rates, poor CR rate (without BMT) and high relapse rate (with BMT). Furthermore, relative luciferase activity of methylated MDR1 at promoter -50 region was significantly higher than that of the unmethylated. In addition, MDR1 in K562 cells elevated significantly after 5-Aza-dC treatment. In summary, MDR1 promoter hypomethylation conferred its up-regulation and indicated poor prognosis in patients with and without BMT.
Предметные рубрики MeSH
ATP Binding Cassette Transporter, Subfamily BATP Binding Cassette Transporter, Subfamily B, Member 1AdolescentAdultBase SequenceBone Marrow TransplantationChildChild, PreschoolDNA MethylationDNA PrimersFemaleFlow CytometryHematologic NeoplasmsHumansMaleMiddle AgedPrognosisPromoter Regions, GeneticReverse Transcriptase Polymerase Chain ReactionUp-RegulationYoung Adult