Archives of pediatrics & adolescent medicine · 1996
Optical immunoassay for rapid detection of group A beta-hemolytic streptococci. Should culture be replaced?
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Schlager T A, Hayden G A, Woods W A, Dudley S M, Hendley J O
Аннотация
OBJECTIVE: To evaluate an optical immunoassay rapid antigen test to detect group A beta-hemolytic streptococci directly from throat swab specimens.
DESIGN: Criterion standard with "blinded" comparison. Double-swab pharyngeal samples were obtained; one swab was cultured and the other was used for the rapid antigen test.
SETTING: Microbiology laboratory in a primary care center at a university teaching hospital.
PATIENTS: Two hundred sixty-two outpatients with pharyngitis.
MAIN OUTCOME MEASURE: The results of the optical immunoassay rapid antigen test were compared with results of standard aerobic culture.
RESULTS: Sixty-two (24%) of 262 samples were culture-positive; 48 of these 62 were positive by the optical immunoassay rapid test (sensitivity, 77%). Of the 200 culture-negative samples, seven (4%) were positive by the rapid test (specificity, 96%).
CONCLUSION: The optical immunoassay performed well, but like other rapid tests, is not sensitive enough to replace standard culture for detection of group A beta-hemolytic streptococci.
DESIGN: Criterion standard with "blinded" comparison. Double-swab pharyngeal samples were obtained; one swab was cultured and the other was used for the rapid antigen test.
SETTING: Microbiology laboratory in a primary care center at a university teaching hospital.
PATIENTS: Two hundred sixty-two outpatients with pharyngitis.
MAIN OUTCOME MEASURE: The results of the optical immunoassay rapid antigen test were compared with results of standard aerobic culture.
RESULTS: Sixty-two (24%) of 262 samples were culture-positive; 48 of these 62 were positive by the optical immunoassay rapid test (sensitivity, 77%). Of the 200 culture-negative samples, seven (4%) were positive by the rapid test (specificity, 96%).
CONCLUSION: The optical immunoassay performed well, but like other rapid tests, is not sensitive enough to replace standard culture for detection of group A beta-hemolytic streptococci.