Cell · 2023
A bat MERS-like coronavirus circulates in pangolins and utilizes human DPP4 and host proteases for cell entry.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Chen Jing, Yang Xinglou, Si Haorui, Gong Qianchun, Que Tengcheng, Li Jing, Li Yang, Wu Chunguang, Zhang Wei, Chen Ying, Luo Yun, Zhu Yan, Li Bei, Luo Dongsheng, Hu Ben, Lin Haofeng, Jiang Rendi, Jiang Tingting, Li Qian, Liu Meiqin, Xie Shizhe, Su Jia, Zheng Xiaoshuang, Li Ang, Yao Yulin, Yang Yong, Chen Panyu, Wu Aiqiong, He Meihong, Lin Xinhua, Tong Yigang, Hu Yanling, Shi Zheng-Li, Zhou Peng
Аннотация
It is unknown whether pangolins, the most trafficked mammals, play a role in the zoonotic transmission of bat coronaviruses. We report the circulation of a novel MERS-like coronavirus in Malayan pangolins, named Manis javanica HKU4-related coronavirus (MjHKU4r-CoV). Among 86 animals, four tested positive by pan-CoV PCR, and seven tested seropositive (11 and 12.8%). Four nearly identical (99.9%) genome sequences were obtained, and one virus was isolated (MjHKU4r-CoV-1). This virus utilizes human dipeptidyl peptidase-4 (hDPP4) as a receptor and host proteases for cell infection, which is enhanced by a furin cleavage site that is absent in all known bat HKU4r-CoVs. The MjHKU4r-CoV-1 spike shows higher binding affinity for hDPP4, and MjHKU4r-CoV-1 has a wider host range than bat HKU4-CoV. MjHKU4r-CoV-1 is infectious and pathogenic in human airways and intestinal organs and in hDPP4-transgenic mice. Our study highlights the importance of pangolins as reservoir hosts of coronaviruses poised for human disease emergence.