Bioactive Spirocyclic C-Arylglycosides From the Fungal-Fungal Co-Culture of Pestalotiopsis sp. and Nigrospora sp.

INTRODUCTION

In this study, two new papulacandin analogs (1-2), together with 12 known compounds (3-14), were identified from the co-culture of two endophytic fungi, Pestalotiopsis sp. J025 and Nigrospora sp. Wn-2-2, which were isolated from Wikstroemia nutans Champ. ex Benth.

OBJECTIVES

The aim of this study is to investigate the antifungal activity of papulacandins and other secondary metabolites from the co-culture of two endophytic fungi.

MATERIAL AND

METHODS

Their chemical structures, including relative configurations, were elucidated using extensive spectroscopic analyses, including HRESIMS, 1D and 2D NMR, ECD calculations, and quantum mechanical-NMR calculations, as well as comparison with reported analogs.

RESULTS

Compound 3 demonstrated promising in vitro antifungal activity against fluconazole-resistant Candida albicans with a minimum inhibitory concentration in 50% isolates (MIC50) of 0.57 ± 0.01 μM.

CONCLUSION

These findings demonstrate that the papulacandin family is a promising resource for the development of antifungal agents against invasive Candida infections.