[Research for the antiapoptotic mechanisms of bone marrow mesenchymal stem cells in the treatment of brain ischemia stroke].

OBJECTIVE

To evaluate the anti-apoptosis role of bone marrow mesenchymal stem cells (BMMCs) transplantation to cell cerebral brain ischemia mice.

METHODS

BMMCs were separated through Ficoll from bone marrow, after amplified in vitro, flow cytometry was used to identify the surface markers. Then cells were transplanted into Middle cerebral artery occlusion (MACO) mice, in situ cell death detection kit and Western blot were used to examine the cell apoptosis. Immunofluorescence and Western blot were used to detect the expression of eNOS, ICAM-1, CD31 which are related to the repair of vascular endothelial cells.

RESULTS

After BMMCs transplantation, the number of apoptosis cells was decreased from (78.2±1.4) to (12.8±3.0), P<0.05. The expression of eNOS (80.0±6.2 vs 31.2±1.6, P<0.01) and CD31 (85±3 vs 45±5, P<0.01), were higher than MACO, while ICAM-1 was lower than MACO (34.1±2.2 vs 85.2±2.8, P<0.01).

CONCLUSION

BMMCs reduce the cell apoptosis of ischemia mice through regulate the expression of vascular endothelial cells relate genes.