Emerging infectious diseases · 2003
Molecular characterization of a non-Babesia divergens organism causing zoonotic babesiosis in Europe.
Клинический случайСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Herwaldt Barbara L, Cacciò Simone, Gherlinzoni Filippo, Aspöck Horst, Slemenda Susan B, Piccaluga PierPaolo, Martinelli Giovanni, Edelhofer Renate, Hollenstein Ursula, Poletti Giovanni, Pampiglione Silvio, Löschenberger Karin, Tura Sante, Pieniazek Norman J
Аннотация
In Europe, most reported human cases of babesiosis have been attributed, without strong molecular evidence, to infection with the bovine parasite Babesia divergens. We investigated the first known human cases of babesiosis in Italy and Austria, which occurred in two asplenic men. The complete 18S ribosomal RNA (18S rRNA) gene was amplified from specimens of their whole blood by polymerase chain reaction (PCR). With phylogenetic analysis, we compared the DNA sequences of the PCR products with those for other Babesia spp. The DNA sequences were identical for the organism from the two patients. In phylogenetic analysis, the organism clusters with B. odocoilei, a parasite of white-tailed deer; these two organisms form a sister group with B. divergens. This evidence indicates the patients were not infected with B. divergens but with an organism with previously unreported molecular characteristics for the 18S rRNA gene.