Shanghai kou qiang yi xue = Shanghai journal of stomatology · 2011
[Establishment of a correlated cell model of oral lichen planus in vitro].
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Li Qin, Du Guan-huan, Tang Guo-yao
Аннотация
PURPOSE: To establish a correlated cocultured model of keratinocytes and T lymphocytes in vitro to simulate the immune response circumstance of oral lichen planus(OLP) lesions.
METHODS: Keratinocytes prepared with enzyme digestion from normal oral mucosa and T lymphocytes prepared with negative isolation procedure by magnetic beads were co-cultured. Proliferation of T lymphocytes in cocultured cells was detected using MTT assay and the expression of IFN-γ was detected by ELISA. All the results were analyzed with independent samples t test using SAS6.12 software package.
RESULTS: The proliferation level of T lymphocytes in the cocultured group of T lymphocytes and keratinocytes which expressed PD-L1 and PD-L2 was higher than that in the group of T lymphocytes (P < 0.05). The expression of IFN-γ in the cocultured group was significantly higher than that in the group of T lymphocytes (P < 0.01).
CONCLUSIONS: The cocultured model of T lymphocytes and keratinocytes which express PD-L1 and PD-L2 can simulate the immune response circumstance of OLP lesions to a certain extent. Supported by National Natural Science Foundation of China (30872888),Research Fund of Science and Technology Commission of Shanghai Municipality(08DZ2271100) and Shanghai Leading Academic Discipline Project (S30206).
METHODS: Keratinocytes prepared with enzyme digestion from normal oral mucosa and T lymphocytes prepared with negative isolation procedure by magnetic beads were co-cultured. Proliferation of T lymphocytes in cocultured cells was detected using MTT assay and the expression of IFN-γ was detected by ELISA. All the results were analyzed with independent samples t test using SAS6.12 software package.
RESULTS: The proliferation level of T lymphocytes in the cocultured group of T lymphocytes and keratinocytes which expressed PD-L1 and PD-L2 was higher than that in the group of T lymphocytes (P < 0.05). The expression of IFN-γ in the cocultured group was significantly higher than that in the group of T lymphocytes (P < 0.01).
CONCLUSIONS: The cocultured model of T lymphocytes and keratinocytes which express PD-L1 and PD-L2 can simulate the immune response circumstance of OLP lesions to a certain extent. Supported by National Natural Science Foundation of China (30872888),Research Fund of Science and Technology Commission of Shanghai Municipality(08DZ2271100) and Shanghai Leading Academic Discipline Project (S30206).