Do commonly used herbicides (atrazine and glyphosate) have the potential to impair the contractions, prostaglandin releasing and conducting of oxytocin signal at the bovine cervix in vitro?

Glyphosate (Gly) and atrazine (Atr) are among the most commonly used herbicides in global agriculture. It was previously shown that both Atr and Gly impair the ovarian and uterine secretion of regulators of myometrial motility (oxytocin (OT) or prostaglandins (PGs)) in cows, and Atr can also decrease the force of contractions in strips from the uterine horn. Hence, the aim of this study was to compare the effects of Atr and Gly on the motor and secretory function of the bovine cervix in vitro as well as receptivity and signal transduction in cervical cell cultures. Cervical strips or cells obtained from cows before ovulation were treated with environmental doses of Atr or Gly (0.1-10 ng/ml) since these herbicides exerted no cytotoxic effect at a dose of 100 ng/ml. Only Atr increased the force of cervical contractions, while both Atr and Gly decreased the secretion of prostaglandins (PGs) without disturbing their synthesis. Moreover, Atr decreased the mRNA expression and protein level of oxytocin receptor (OTR), while Gly increased OTR protein levels. Both Atr and Gly decreased the contents of gap junction proteins (GAPs), Atr decreased the contents of second messengers (diacylglycerol - DAG, inositol-tris-phosphate - IP3), and Gly decreased the level of myosin light chain kinase (MLCK) but increased DAG levels. Atr directly enhanced the cervical strips contractions. Both herbicides disturbed cellular signalling and inhibited PGs secretion. It suggest that Atr and Gly have the potential to impair the activity of cervical cells in vitro, which might be followed by failure of maintenance with gestation.