Bioanalysis · 2016
High-throughput method for the quantitation of metabolites and co-factors from homocysteine-methionine cycle for nutritional status assessment.
СтатьяВалидационное исследованиеНазвание и аннотация: Перевод пока не подготовлен
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Da Silva Laeticia, Collino Sebastiano, Cominetti Ornella, Martin Francois-Pierre, Montoliu Ivan, Moreno Sergio Oller, Corthesy John, Kaput Jim, Kussmann Martin, Monteiro Jacqueline Pontes, Guiraud Seu Ping
Аннотация
AIM: There is increasing interest in the profiling and quantitation of methionine pathway metabolites for health management research. Currently, several analytical approaches are required to cover metabolites and co-factors.
RESULTS: We report the development and the validation of a method for the simultaneous detection and quantitation of 13 metabolites in red blood cells. The method, validated in a cohort of healthy human volunteers, shows a high level of accuracy and reproducibility.
CONCLUSION: This high-throughput protocol provides a robust coverage of central metabolites and co-factors in one single analysis and in a high-throughput fashion. In large-scale clinical settings, the use of such an approach will significantly advance the field of nutritional research in health and disease.
RESULTS: We report the development and the validation of a method for the simultaneous detection and quantitation of 13 metabolites in red blood cells. The method, validated in a cohort of healthy human volunteers, shows a high level of accuracy and reproducibility.
CONCLUSION: This high-throughput protocol provides a robust coverage of central metabolites and co-factors in one single analysis and in a high-throughput fashion. In large-scale clinical settings, the use of such an approach will significantly advance the field of nutritional research in health and disease.