The Journal of nutrition · 2025
Inverse Association between Serum Vitamin B12 Level and Abundance of Potential B12-Producing Gut Microbes in Indian Children.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Chandel Nisha, Patel Priyansh, Somvanshi Pramod R, Verma Anil Kumar, Thakur Vivek
Аннотация
BACKGROUND: The human gut microbiome is a natural source of essential micronutrients like B vitamins, which are used by both the host and other community members. The prevalence and abundance of known B-vitamin producers and B-vitamin biosynthesis pathways have already been reported in gut microbiome cohorts of a few countries including India.
OBJECTIVES: This study tested whether the presence of B-vitamin producers/biosynthetic pathways associates with serum B-vitamin levels, taking vitamin B12 as a case example.
METHODS: Fecal samples were collected from non-deficient (serum vitamin B12 level > 210 pg/mL; n = 29) and vitamin B12 deficient (serum vitamin B12 level < 210 pg/mL; n = 30) children from a tribal region of central India. Whole metagenomic DNA was extracted, sequenced, and analyzed for taxonomic profiling and diversity comparisons. Differentially abundant taxa between two groups were identified. The prevalence and abundance of potential vitamin B12 producers were compared, and their association with serum vitamin B12 level was established.
RESULTS: A comparison of within-sample diversity between the two groups did not show any difference; however, between-sample diversity was significantly less in the vitamin B12-deficient group. Differential abundance testing also showed different microbiome structure in the vitamin B12-deficient group, where a higher abundance of vitamin B12 transporter-carrying Bacteroides thetaiotaomicron, a few pathogenic species, and ten known vitamin B12 producers was observed. Potential vitamin B12 producers were also significantly prevalent and abundant in the deficient group. Their cumulative abundance was also significantly higher in the deficient group and showed a negative association with serum vitamin B12 levels.
CONCLUSIONS: A higher abundance of potential vitamin B12 producers in the deficient group suggest an adaptive mechanism by the gut microbiome to meet the community's vitamin B12 requirements, by selectively promoting the growth of vitamin B12 producers, but causality remains to be proved.
OBJECTIVES: This study tested whether the presence of B-vitamin producers/biosynthetic pathways associates with serum B-vitamin levels, taking vitamin B12 as a case example.
METHODS: Fecal samples were collected from non-deficient (serum vitamin B12 level > 210 pg/mL; n = 29) and vitamin B12 deficient (serum vitamin B12 level < 210 pg/mL; n = 30) children from a tribal region of central India. Whole metagenomic DNA was extracted, sequenced, and analyzed for taxonomic profiling and diversity comparisons. Differentially abundant taxa between two groups were identified. The prevalence and abundance of potential vitamin B12 producers were compared, and their association with serum vitamin B12 level was established.
RESULTS: A comparison of within-sample diversity between the two groups did not show any difference; however, between-sample diversity was significantly less in the vitamin B12-deficient group. Differential abundance testing also showed different microbiome structure in the vitamin B12-deficient group, where a higher abundance of vitamin B12 transporter-carrying Bacteroides thetaiotaomicron, a few pathogenic species, and ten known vitamin B12 producers was observed. Potential vitamin B12 producers were also significantly prevalent and abundant in the deficient group. Their cumulative abundance was also significantly higher in the deficient group and showed a negative association with serum vitamin B12 levels.
CONCLUSIONS: A higher abundance of potential vitamin B12 producers in the deficient group suggest an adaptive mechanism by the gut microbiome to meet the community's vitamin B12 requirements, by selectively promoting the growth of vitamin B12 producers, but causality remains to be proved.