The PcbZIP44-PcWRKY70 Module Mediates Volatile Ester Biosynthesis Via PcAAT1 Transcriptional Regulation during Pear Postharvest Ripening.

Aroma profoundly influences the flavor profile and commercial appeal of fruits. While the biosynthetic pathways of volatiles are well understood, the regulatory mechanisms governing their transcription remain unclear. This study used 'Starkrimson' fruits (Pyrus communis L.) to explore molecular mechanisms of ester biosynthesis. Integration of gas chromatography-mass spectrometry analysis and RNA sequencing data revealed that the alcohol acyltransferase PcAAT1 is involved in ester synthesis. Experiments confirmed transcription factor PcbZIP44 as its upstream activator, and its physical interaction with PcWRKY70, forming a complex that synergistically enhances PcAAT1 transcription, affecting pear ester biosynthesis. Our findings clarify a novel transcriptional regulatory module, PcbZIP44-PcWRKY70, whose formed complex synergistically and significantly enhances the expression of PcAAT1, thereby affecting ester biosynthesis in pears. This elucidated PcbZIP44-PcWRKY70 module expands our understanding of transcriptional complexes in aroma regulation and presents potential targets for pear flavor enhancement.