Validation of the novel Martin method for LDL cholesterol estimation.

INTRODUCTION

Accurate determination of low-density lipoprotein cholesterol (LDL) is important for coronary heart disease (CHD) risk assessment among others. A low-cost method used is the Friedewald equation, calculating LDL from total cholesterol after subtracting high-density lipoprotein cholesterol (HDL) and triglycerides divided by 5. A new calculation method has been proposed where the value of 5 is not fixed but depends on the values of the other parameters.

RESULTS

We validated this method in a Greek population sample, by comparing direct LDL, the Friedewald equation and the novel method. Some clinical laboratories use the direct determination when TG > 200 mg/dl (2.26 mmol/lt). We performed segmented linear regression to check if this value makes sense. Bayesian linear regression was performed to compare the direct determination to the Friedewald and novel one.

CONCLUSIONS

We found that TG > 200 mg/dl is a sensible threshold value since it is a saddle point for the standard error of the regression. For Bayesian linear regression, the results were inconclusive. When the LDL values were used for classification of CHD, it turned out that the novel method was better than the Friedewald equation at correctly classifying LDL levels for CHD risk assessment.