Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control · 2015
[Isolation and identification of macrophages from liver of Microtus fortis].
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Hu Yuan, Sun Lei, Xu Yu-xin, Cao Jian-ping
Аннотация
OBJECTIVE: To separate and purify intrahepatic macrophages from Microtus fortis (Mf) and identify its phagocytosis.
METHODS: The intrahepatic macrophages from Mf were separated and purified by perfusion, collagenase digestion and density gradient centrifugation. The function of the cells was identified by FACS analysis and ink phagocytosis activity.
RESULTS: The macrophage cells from the liver of Mf were obtained. These cells were bright and circular, and grew adhering to the wall. The proportion of the living cells was 95%. The binding rate of these cells from Mf with anti-mouse CD14 antibody (Clone, Sa2-8) was about 50% of the rate of macrophage from C57BL/6 mice with this monoclonal antibody. The result of ink-phagocytosis experiment of macrophage cells from the liver of Mf was positive.
CONCLUSION: The method above mentioned is useful to separate and purify macrophage from the liver of Mf. The study builds the foundation for further research on macrophages of Mf against Schistosomajaponicum.
METHODS: The intrahepatic macrophages from Mf were separated and purified by perfusion, collagenase digestion and density gradient centrifugation. The function of the cells was identified by FACS analysis and ink phagocytosis activity.
RESULTS: The macrophage cells from the liver of Mf were obtained. These cells were bright and circular, and grew adhering to the wall. The proportion of the living cells was 95%. The binding rate of these cells from Mf with anti-mouse CD14 antibody (Clone, Sa2-8) was about 50% of the rate of macrophage from C57BL/6 mice with this monoclonal antibody. The result of ink-phagocytosis experiment of macrophage cells from the liver of Mf was positive.
CONCLUSION: The method above mentioned is useful to separate and purify macrophage from the liver of Mf. The study builds the foundation for further research on macrophages of Mf against Schistosomajaponicum.