[Isolation and identification of macrophages from liver of Microtus fortis].

OBJECTIVE

To separate and purify intrahepatic macrophages from Microtus fortis (Mf) and identify its phagocytosis.

METHODS

The intrahepatic macrophages from Mf were separated and purified by perfusion, collagenase digestion and density gradient centrifugation. The function of the cells was identified by FACS analysis and ink phagocytosis activity.

RESULTS

The macrophage cells from the liver of Mf were obtained. These cells were bright and circular, and grew adhering to the wall. The proportion of the living cells was 95%. The binding rate of these cells from Mf with anti-mouse CD14 antibody (Clone, Sa2-8) was about 50% of the rate of macrophage from C57BL/6 mice with this monoclonal antibody. The result of ink-phagocytosis experiment of macrophage cells from the liver of Mf was positive.

CONCLUSION

The method above mentioned is useful to separate and purify macrophage from the liver of Mf. The study builds the foundation for further research on macrophages of Mf against Schistosomajaponicum.