Muscle & nerve · 2018
Altered bone-regulating myokine expression in skeletal muscle Of Duchenne muscular dystrophy mouse models.
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Zhou Shumin, Qian Baoli, Wang Ling, Zhang Changqing, Hogan Macalus V, Li Hongshuai
Аннотация
INTRODUCTION: Duchenne muscular dystrophy (DMD) has been well characterized as a disease that affects both skeletal muscle and bone. The pathophysiology responsible for the deficits in bone tissue is still unclear.
METHODS: Quantitative reverse-transcription polymerase chain reaction and Western blot analyses of known myokines from skeletal muscle were performed on dystrophic mouse models and wild-type (WT) controls to identify differentially expressed bone-regulating myokines.
RESULTS: Twenty-four of 43 myokine genes demonstrated significantly different mRNA expression in the skeletal muscles of dystrophic mice when compared with muscles of WT mice. Several differently expressed bone-regulating myokine genes were identified, and their protein levels were also verified by Western blot.
CONCLUSIONS: Dystrophic skeletal muscle demonstrated a significantly altered myokine gene expression profile. mRNA and protein levels of several bone-regulating myokines were significantly altered in dystrophic skeletal muscle, which suggests pathological role of bone-regulating myokines on bone homeostasis in DMD. Muscle Nerve 58: 573-582, 2018.
METHODS: Quantitative reverse-transcription polymerase chain reaction and Western blot analyses of known myokines from skeletal muscle were performed on dystrophic mouse models and wild-type (WT) controls to identify differentially expressed bone-regulating myokines.
RESULTS: Twenty-four of 43 myokine genes demonstrated significantly different mRNA expression in the skeletal muscles of dystrophic mice when compared with muscles of WT mice. Several differently expressed bone-regulating myokine genes were identified, and their protein levels were also verified by Western blot.
CONCLUSIONS: Dystrophic skeletal muscle demonstrated a significantly altered myokine gene expression profile. mRNA and protein levels of several bone-regulating myokines were significantly altered in dystrophic skeletal muscle, which suggests pathological role of bone-regulating myokines on bone homeostasis in DMD. Muscle Nerve 58: 573-582, 2018.
Предметные рубрики MeSH
AnimalsBlotting, WesternBone Diseases, MetabolicBone and BonesCase-Control StudiesCell Adhesion MoleculesCytokinesDisease Models, AnimalFemurFibroblast Growth FactorsFibronectinsInterleukin-6Leukemia Inhibitory FactorLower ExtremityLumbar VertebraeMiceMice, Inbred mdxMuscle, SkeletalMuscular Dystrophy, DuchenneMyostatinOsteonectinRANK LigandRNA, MessengerReverse Transcriptase Polymerase Chain ReactionX-Ray Microtomography