PloS one · 2026
Repeated dosing of myrrh, chamomile extract, and coffee charcoal reveals potential health-beneficial effects in patients with irritable bowel syndrome in the M-SHIME simulator.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Wonnemann Meinolf, Lipowicz Bartosz, Duysburgh Cindy, Rotsaert Chloë, Verstrepen Lynn, Marzorati Massimo, Langhorst Jost
Аннотация
OBJECTIVE: To define the potential functional roles of a herbal preparation of myrrh, chamomile extract, and coffee charcoal in patients suffering from diarrhea dominant irritable bowel syndrome (IBS-D).
METHODS: The study utilized the Mucosal Simulator of the Human Intestinal Microbial Environment (M-SHIME®) with proximal (PC) and distal colon (DC) compartments and fecal samples from four IBS-D donors. Eight-day (d) repeated dosing with the herbal product (6 tablets/day) was initiated compared to a negative control. Changes in microbial metabolism and community composition were assessed, and colonic ferments were evaluated for their effects on intestinal barrier permeability and cytokine production in a Caco-2/THP-1 co-culture model.
RESULTS: Product treatment significantly increased gas pressure versus negative control, indicating microbial fermentative activity. Product supplementation significantly increased proximal acetate (d3, d5), propionate (d3), and butyrate (d5, d8) levels (p < 0.05 for all), while no significant changes were observed distally. Ammonium levels were significantly elevated following product supplementation in PC (d3, d5, d8; p < 0.05) and DC (d8; p < 0.01), though remained within physiological range. Repeated dosing enriched members of Bifidobacteriaceae, Bacteroidota, Lachnospiraceae, and Butyricicoccus versus negative control. Treated colonic ferments had a protective effect on intestinal membrane integrity (DC; p < 0.001) and positive immunomodulatory effects (increased IL-10, PC and DC [both p < 0.0001], and IL-6, PC [p < 0.001] and DC [p < 0.01]) in Caco-2/THP-1 co-cultures.
CONCLUSIONS: Treatment with a herbal preparation of myrrh, chamomile extract, and coffee charcoal showed a potential beneficial effect on the microbiota of patients with IBS-D in vitro, suggesting further exploration of its efficacy in IBS-D and other chronic gastrointestinal disorders.
METHODS: The study utilized the Mucosal Simulator of the Human Intestinal Microbial Environment (M-SHIME®) with proximal (PC) and distal colon (DC) compartments and fecal samples from four IBS-D donors. Eight-day (d) repeated dosing with the herbal product (6 tablets/day) was initiated compared to a negative control. Changes in microbial metabolism and community composition were assessed, and colonic ferments were evaluated for their effects on intestinal barrier permeability and cytokine production in a Caco-2/THP-1 co-culture model.
RESULTS: Product treatment significantly increased gas pressure versus negative control, indicating microbial fermentative activity. Product supplementation significantly increased proximal acetate (d3, d5), propionate (d3), and butyrate (d5, d8) levels (p < 0.05 for all), while no significant changes were observed distally. Ammonium levels were significantly elevated following product supplementation in PC (d3, d5, d8; p < 0.05) and DC (d8; p < 0.01), though remained within physiological range. Repeated dosing enriched members of Bifidobacteriaceae, Bacteroidota, Lachnospiraceae, and Butyricicoccus versus negative control. Treated colonic ferments had a protective effect on intestinal membrane integrity (DC; p < 0.001) and positive immunomodulatory effects (increased IL-10, PC and DC [both p < 0.0001], and IL-6, PC [p < 0.001] and DC [p < 0.01]) in Caco-2/THP-1 co-cultures.
CONCLUSIONS: Treatment with a herbal preparation of myrrh, chamomile extract, and coffee charcoal showed a potential beneficial effect on the microbiota of patients with IBS-D in vitro, suggesting further exploration of its efficacy in IBS-D and other chronic gastrointestinal disorders.