IEEE transactions on bio-medical engineering · 2026
Fiber-Less, Large-Scale Opto-Electrophysiology Interface for Micro-Scale Interaction of Multiple Brain Regions.
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Oh Sungjin, Ruiz Jose Roberto Lopez, Kim Kanghwan, Slager Nathan, Ko Eunah, Voroslakos Mihaly, Song Hyunsoo, Chen Wangbo, Park Sung-Yun, Yoon Euisik
Аннотация
OBJECTIVE: Recent neuroscientific research craves for understanding sophisticated brain networks formed by neuron ensembles across multiple regions. An ideal way to unveil the complex connectome is bidirectionally interacting (simultaneous recording and stimulation) with neurons at high spatiotemporal resolutions. Existing CMOS recording probes cannot provide micro-scale interactions with limited stimulation capability. Although optogenetics can achieve neuron-specific stimulation, conventional methods using optic fibers illuminate a large volume of tissue, resulting in unspecific perturbations. While our previous studies demonstrated micro-LED (µLED)-based optoelectrode for localized stimulation and recording, this work advances them into a fully integrated headstage combining the optoelectrode, CMOS IC, and flexible interposer for miniaturized implementation. The proposed system enables micro-scale interactions with high spatiotemporal precision through densely packed 256-neuron-size recording and 128-soma-size fiber-less opto-stimulation across multiple brain regions.
METHODS: Such high resolutions yet wide coverage is achieved by (1) advanced micromachining techniques integrating recording electrodes and µLEDs, (2) micro-second, independent 384-channel interaction via a low-power, area-efficient circuit, and (3) compact and reliable polyimide-cable-based hybrid assembly.
RESULTS: A compact (23.8×28.8 mm2) and lightweight (3.5-gram) headstage achieved the highest reported channel density in area (0.56 channels/mm2) and weight (109.71 channels/gram). A single acute in vivo experiment on a transgenic mouse identified >160 isolated pyramidal neurons and narrow/wide interneurons in the dorsal hippocampus, with local and broad-range effects from focal optogenetic stimulation.
CONCLUSION AND SIGNIFICANCE: We implemented the hybrid integrated, large-scale opto-electrophysiology interface prototype and verified its feasibility in vivo, representing the first fully integrated platform extending our µLED-based probes into a complete system.
METHODS: Such high resolutions yet wide coverage is achieved by (1) advanced micromachining techniques integrating recording electrodes and µLEDs, (2) micro-second, independent 384-channel interaction via a low-power, area-efficient circuit, and (3) compact and reliable polyimide-cable-based hybrid assembly.
RESULTS: A compact (23.8×28.8 mm2) and lightweight (3.5-gram) headstage achieved the highest reported channel density in area (0.56 channels/mm2) and weight (109.71 channels/gram). A single acute in vivo experiment on a transgenic mouse identified >160 isolated pyramidal neurons and narrow/wide interneurons in the dorsal hippocampus, with local and broad-range effects from focal optogenetic stimulation.
CONCLUSION AND SIGNIFICANCE: We implemented the hybrid integrated, large-scale opto-electrophysiology interface prototype and verified its feasibility in vivo, representing the first fully integrated platform extending our µLED-based probes into a complete system.