The Journal of infectious diseases · 2008
Increased expression of type-1 fimbriae by nonpathogenic Escherichia coli 83972 results in an increased capacity for catheter adherence and bacterial interference.
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Trautner Barbara W, Cevallos Manuel E, Li Huaiguang, Riosa Sarah, Hull Richard A, Hull Sheila I, Tweardy David J, Darouiche Rabih O
Аннотация
BACKGROUND: In vitro, urinary catheter colonization by avirulent Escherichia coli 83972 impedes subsequent catheter colonization by a variety of uropathogenic organisms. However, E. coli 83972 shows a low efficacy of adherence to silicone urinary catheter material, possibly because the fim operon encoding adhesive type 1 fimbriae is incomplete. We hypothesized that improving the catheter adherence of E. coli 83972 would improve its bacterial interference properties.
METHODS: We created adhesive mutants by transforming wild-type E. coli 83972 with fim(+) plasmids. Adherence to urinary catheters and ability to prevent uropathogenic E. coli from colonizing urinary catheters were studied by use of a sonication assay.
RESULTS: The addition of a single-copy fim(+) plasmid increased adherence to urinary catheters 10-fold, and addition of an 18-copy fim(+) plasmid increased adherence 100-fold. The more adherent 18-copy fim(+) plasmid strain was more effective at blocking catheter colonization by pathogenic E. coli than was the wild-type parental strain. Neither Deltafim nor fim(+) E. coli 83972 adhered to shed urinary epithelial cells.
CONCLUSIONS: Our results indicate that improving urinary catheter adherence augments the bacterial interference capabilities of benign E. coli 83972. Increased expression of type-1 fimbriae may enhance bacterial interference without conferring virulence on E. coli 83972.
METHODS: We created adhesive mutants by transforming wild-type E. coli 83972 with fim(+) plasmids. Adherence to urinary catheters and ability to prevent uropathogenic E. coli from colonizing urinary catheters were studied by use of a sonication assay.
RESULTS: The addition of a single-copy fim(+) plasmid increased adherence to urinary catheters 10-fold, and addition of an 18-copy fim(+) plasmid increased adherence 100-fold. The more adherent 18-copy fim(+) plasmid strain was more effective at blocking catheter colonization by pathogenic E. coli than was the wild-type parental strain. Neither Deltafim nor fim(+) E. coli 83972 adhered to shed urinary epithelial cells.
CONCLUSIONS: Our results indicate that improving urinary catheter adherence augments the bacterial interference capabilities of benign E. coli 83972. Increased expression of type-1 fimbriae may enhance bacterial interference without conferring virulence on E. coli 83972.