Journal of equine veterinary science · 2025
Effects of seminal plasma on uterine polymorphonuclear dynamics and fertility of jennies inseminated with glycerol-free, frozen-thawed donkey semen.
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Vargas Jeimmy Hernández, Zarco Luis, Salinas Elizabeth Morales, Urías-Castro Cristian, Boeta Myriam
Аннотация
BACKGROUND: Insemination of jennies with frozen-thawed donkey semen usually causes an intense uterine inflammation and results in low fertility.
OBJECTIVES: To evaluate the effects of seminal plasma on the time course of endometrial inflammation after insemination of jennies with donkey semen that was frozen using formamides as cryoprotective agents.
METHODS: Jennies were inseminated with fresh semen (FS, n = 14), frozen-thawed semen (FTS, n = 12) or frozen-thawed semen plus seminal plasma (FTS-SP, n = 13), and uterine cytology was evaluated at 8, 24 and 48 h post-insemination.
RESULTS: At 8 h post-insemination the percentage of polymorphonuclear cells (%PMN) was higher (P < 0.05) in the FTS group (90.7 ± 4.6) than in the FS group (68.9 ± 4.3), while jennies in the FTS-SP had intermediate levels (82.7 ± 4.5). At 24 h the %PMN was higher (P < 0.05) in jennies in the FTS-SP group (84.8 ± 4.5) than in the other groups. However, a faster decline in %PMN from 24 h to 48 h post-insemination in the FTS-SP group resulted in no difference between groups at 48 h. All groups still had mean %PMNs above 50 % at 48 h post-insemination, but the females that became pregnant had faster clearance rates from 24 h to 48 h post-insemination, resulting in lower %PMNs (44.4 ± 3.8) than those in the non-pregnant jennies (64.8 ± 3.6) at 48 h post-insemination (P<0.05).
CONCLUSION: Seminal plasma induced faster uterine polymorphonuclear clearance after 24 h post-insemination in jennies inseminated with frozen-thawed donkey semen, and this effect could be related to improved fertility.
OBJECTIVES: To evaluate the effects of seminal plasma on the time course of endometrial inflammation after insemination of jennies with donkey semen that was frozen using formamides as cryoprotective agents.
METHODS: Jennies were inseminated with fresh semen (FS, n = 14), frozen-thawed semen (FTS, n = 12) or frozen-thawed semen plus seminal plasma (FTS-SP, n = 13), and uterine cytology was evaluated at 8, 24 and 48 h post-insemination.
RESULTS: At 8 h post-insemination the percentage of polymorphonuclear cells (%PMN) was higher (P < 0.05) in the FTS group (90.7 ± 4.6) than in the FS group (68.9 ± 4.3), while jennies in the FTS-SP had intermediate levels (82.7 ± 4.5). At 24 h the %PMN was higher (P < 0.05) in jennies in the FTS-SP group (84.8 ± 4.5) than in the other groups. However, a faster decline in %PMN from 24 h to 48 h post-insemination in the FTS-SP group resulted in no difference between groups at 48 h. All groups still had mean %PMNs above 50 % at 48 h post-insemination, but the females that became pregnant had faster clearance rates from 24 h to 48 h post-insemination, resulting in lower %PMNs (44.4 ± 3.8) than those in the non-pregnant jennies (64.8 ± 3.6) at 48 h post-insemination (P<0.05).
CONCLUSION: Seminal plasma induced faster uterine polymorphonuclear clearance after 24 h post-insemination in jennies inseminated with frozen-thawed donkey semen, and this effect could be related to improved fertility.