Glial cells have more important role in tDCS-induced brain activities rather than neurons.

Transcranial Direct Current Stimulation (tDCS) is a non-invasive and sub-threshold method for altering the excitability of neurons to achieve target conditions. Many clinical applications of tDCS are in progress including treatment of neurological disease. Despite the therapeutic and practical potentials of tDCS, the precise mechanism of its impact is still unclear to a large extent. The initial perspective about the effect of tDCS on synaptic plasticity was that electric stimulation of the brain degrades the threshold of neural firing, thus facilitating electrical transmission and improving conductivity between neurons and ultimately leading to changes in the processing of information. However, new results of some experimental studies have changed the initial perspective. Recently, calcium imaging has shown sudden calcium fluctuations, followed by a change in cortical metaplasticity under tDCS. These pieces of evidence pinpoint the important role of astrocytes in describing the effect of tDCS. Astrocytes are glial cells that are traditionally viewed with only a supportive and nutritional role, but recent research expresses that they are active and contribute to processing and regulating synaptic information. We hypothesize that considering astrocytes as an important player in tDCS effect instead of the direct effect on voltage membrane of neurons is closer to the reality of tDCS effect mechanism. This hypothesis is examined here based on the experimental evidence published so far, which indicates astroglia involvement in tDCS-induced activities and results of our proposed computational model.