Comparative secretome and proteome analysis unveils the response mechanism in the phosphorus utilization of Alexandrium pacificum.

Phosphorus (P) acts as a crucial limiting nutrient for the growth of marine phytoplankton cells and the formation of algal blooms. The dinoflagellate Alexandrium pacificum is known for causing frequent and intense blooms in specific estuarine and coastal regions. In this study, we investigated the growth and physiological transformations under conditions characterized by P-deficiency, NaH2PO4, and ATP. For the first time, an integrated comparative analysis of the secretome and proteome was performed to investigate the global protein expression profile of A. pacificum, with 355 and 2308 differentially expressed proteins (DEPs), respectively. The results demonstrated that P-deficiency led to a reduction in growth and notable decreases in metabolic processes in A. pacificum. In P-deficient and ATP groups, the expression of secretory protein alkaline phosphatase A (PhoA) was increased, while intracellular acid phosphatase (ACP) displayed significant upregulation in P-deficient group, indicating that A. pacificum has evolved multiple organic P utilization strategies to adapt to low-P environments. A. pacificum can utilize the intracellular carbohydrate storage pools via glycolysis and the TCA cycle to replenish Calvin cycle intermediates. However, the growth of the ATP and NaH2PO4 groups showed no significant alteration. These results suggest that A. pacificum possesses distinct adaptive strategies towards P-deficiency in the environment and employs specific mechanisms for utilizing organic P, which may be a crucial factor in the formation of blooms in low inorganic P environments.