A novel endophytic Kineococcus from rice against Fusarium culmorum and Fusarium sporotrichioides and whole-genome analysis.

Kineococcus wuchangensis DHX-1 was isolated from rice leaves at the wax-ripening stage and represents a novel species within the genus Kineococcus. In this study, the complete genome of strain DHX-1 was sequenced and assembled, revealing a genome size of 4,808,488 b with a GC content of 73.7%. Antimicrobial assays using the fermentation supernatant demonstrated that strain DHX-1 possesses potential antifungal activity against Fusarium culmorum and Fusarium sporotrichioides. Gas chromatography-mass spectrometry (GC-MS) analyses of volatile components in the strain DHX-1 fermentation broth supernatant, revealed that phenylethyl alcohol and 1,3-benzodioxole, 4-methoxy-6-(2-propenyl) contribute to the antifungal function. Strain DHX-1 exhibits potential as a biocontrol agent, and its discovery expands the known antifungal capabilities and biocontrol applications within the genus Kineococcus.