Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie · 2018
Crocin improves the proliferation and cytotoxic function of T cells in children with acute lymphoblastic leukemia.
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Zhang Kunpeng, Wang Lingzhen, Si Shaoyong, Sun Yan, Pei Wenting, Ming Yan, Sun Lirong
Аннотация
OBJECTIVE: Immunotherapy is important to improve the survival of children with acute lymphoblastic leukemia (ALL). This study aimed to assess the effects of crocin on the proliferation and function of T cells isolated from children with ALL.
METHODS: The mononuclear cells were isolated from peripheral blood of children with ALL and then treated with different final concentrations of crocin. The levels of different cytokines secreted by T cells and the ratio of CD4 and CD8 were measured. Tail DNA% (TDNA), Tail moment (TM), Tail length (TL) and sister chromatid exchange (SCE) were detected to assess DNA damage of T cells.
RESULTS: Crocin significantly promoted T cell proliferation and the secretion of IL-2 and IL-4 in a concentration dependent manner. In addition, crocin increased CD4/CD8 ratio of T subset. Crocin itself caused no significant damage to T cells but reduced DNA damage in T cells treated with Ara-C.
CONCLUSIONS: Crocin could improve the proliferation and cytotoxic function of T cells, and reduce DNA damage caused by Ara-C.
METHODS: The mononuclear cells were isolated from peripheral blood of children with ALL and then treated with different final concentrations of crocin. The levels of different cytokines secreted by T cells and the ratio of CD4 and CD8 were measured. Tail DNA% (TDNA), Tail moment (TM), Tail length (TL) and sister chromatid exchange (SCE) were detected to assess DNA damage of T cells.
RESULTS: Crocin significantly promoted T cell proliferation and the secretion of IL-2 and IL-4 in a concentration dependent manner. In addition, crocin increased CD4/CD8 ratio of T subset. Crocin itself caused no significant damage to T cells but reduced DNA damage in T cells treated with Ara-C.
CONCLUSIONS: Crocin could improve the proliferation and cytotoxic function of T cells, and reduce DNA damage caused by Ara-C.