Head & neck · 2018
Prognostic implications of CD44, NANOG, OCT4, and BMI1 expression in tongue squamous cell carcinoma.
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Rodrigues Maria Fernanda Setúbal Destro, Xavier Flávia Caló de Aquino, Andrade Nathália Paiva, Lopes Camila, Miguita Luiz Lucyene, Sedassari Bruno Tavares, Ibarra Ana Melissa Ccopa, López Rossana Verónica Mendoza, Kliemann Schmerling Claudia, Moyses Raquel Ajub, Tajara da Silva Eloiza Elena, Nunes Fabio Daumas
Аннотация
BACKGROUND: Tongue squamous cell carcinoma (SCC) contains a cell subpopulation referred to as cancer stem cells (CSCs), which are responsible for tumor growth, metastasis, and resistance to chemotherapy and radiotherapy. The CSC markers have been used to isolate these cells and as biomarkers to predict overall survival.
METHODS: The CSC markers CD44, NANOG, OCT4, and BMI1 were investigated using reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) and immunohistochemistry and correlated with clinicopathological parameters.
RESULTS: The CD44 overexpression was associated with disease-related death (P = 0.02) and worst prognosis. NANOG was upregulated in nontumoral margins and associated with T1/T2 classification, lymph node metastasis, and worst prognosis. OCT4 was associated with lymph node metastasis and worst overall survival. BMI1 and CD44v3 were overexpressed in tongue SCC. Coexpression of CD44++ /NANOG++ was associated with worst overall survival when compared with patients with CD44-/+ /NANOG-/+ .
CONCLUSION: The CSC markers might play an important role not only in CSC trait acquisition but also in tongue SCC development and progression.
METHODS: The CSC markers CD44, NANOG, OCT4, and BMI1 were investigated using reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) and immunohistochemistry and correlated with clinicopathological parameters.
RESULTS: The CD44 overexpression was associated with disease-related death (P = 0.02) and worst prognosis. NANOG was upregulated in nontumoral margins and associated with T1/T2 classification, lymph node metastasis, and worst prognosis. OCT4 was associated with lymph node metastasis and worst overall survival. BMI1 and CD44v3 were overexpressed in tongue SCC. Coexpression of CD44++ /NANOG++ was associated with worst overall survival when compared with patients with CD44-/+ /NANOG-/+ .
CONCLUSION: The CSC markers might play an important role not only in CSC trait acquisition but also in tongue SCC development and progression.
Предметные рубрики MeSH
AdultBiomarkers, TumorCarcinoma, Squamous CellEpithelial CellsFemaleHumansHyaluronan ReceptorsImmunohistochemistryKeratinocytesLymphatic MetastasisMaleNanog Homeobox ProteinOctamer Transcription Factor-3Polycomb Repressive Complex 1PrognosisRNA, MessengerReverse Transcriptase Polymerase Chain ReactionTongue NeoplasmsUp-Regulation