Ulusal travma ve acil cerrahi dergisi = Turkish journal of trauma & emergency surgery : TJTES · 2010
The biochemical effectiveness of N-acetylcysteine in experimental spinal cord injury in rats.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Hanci Volkan, Kerimoğlu Alaeddin, Koca Kenan, Başkesen Aykut, Kiliç Kemal, Taştekin Didem
Аннотация
BACKGROUND: In this study, we investigated the biochemical effectiveness of methylprednisolone, N-acetylcysteine (NAC) and methylprednisolone combined with NAC treatment in experimental spinal cord injury in rats.
METHODS: Thirty-two Sprague-Dawley male rats weighing 250-300 g were divided into four groups. Spinal cord injury was created extradurally with an aneurysm clip at the T4-T5 level. Following the trauma, Group C (Control group, n:8) was not given any treatment. Group M (methylprednisolone group, n:8) was treated with 30 mg x kg(-1) methylprednisolone followed by a maintenance dose of 5.4 mg x kg(-1) per hour. Group N (NAC group, n:8) was given 150 mg x kg(-1) NAC. Group MN (methylprednisolone and NAC group, n:8) was given 30 mg x kg(-1) followed by an hourly maintenance dose of 5.4 mg x kg(-1) methylprednisolone and 150 mg x kg(-1) NAC intraperitoneally. Twenty-four hours after the trauma, the rats were decapitated under anesthesia, and their spinal cord samples were taken for biochemical examination.
RESULTS: Mean malonyldialdehyde (MDA) values in Groups M, N and MN were significantly reduced compared to Group C. Mean superoxide dismutase (SOD) values in Groups M, N and MN were significantly higher than in Group C (p<0.05). No difference existed between Groups M and N with respect to mean MDA and SOD values.
CONCLUSION: Methylprednisolone, NAC and methylprednisolone plus NAC treatments have potential biochemical benefits in preventing secondary injury in experimental spinal cord injury in rats.
METHODS: Thirty-two Sprague-Dawley male rats weighing 250-300 g were divided into four groups. Spinal cord injury was created extradurally with an aneurysm clip at the T4-T5 level. Following the trauma, Group C (Control group, n:8) was not given any treatment. Group M (methylprednisolone group, n:8) was treated with 30 mg x kg(-1) methylprednisolone followed by a maintenance dose of 5.4 mg x kg(-1) per hour. Group N (NAC group, n:8) was given 150 mg x kg(-1) NAC. Group MN (methylprednisolone and NAC group, n:8) was given 30 mg x kg(-1) followed by an hourly maintenance dose of 5.4 mg x kg(-1) methylprednisolone and 150 mg x kg(-1) NAC intraperitoneally. Twenty-four hours after the trauma, the rats were decapitated under anesthesia, and their spinal cord samples were taken for biochemical examination.
RESULTS: Mean malonyldialdehyde (MDA) values in Groups M, N and MN were significantly reduced compared to Group C. Mean superoxide dismutase (SOD) values in Groups M, N and MN were significantly higher than in Group C (p<0.05). No difference existed between Groups M and N with respect to mean MDA and SOD values.
CONCLUSION: Methylprednisolone, NAC and methylprednisolone plus NAC treatments have potential biochemical benefits in preventing secondary injury in experimental spinal cord injury in rats.