Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology · 2008
[Expression of CD45RA and CD45RO thymocyte subset and related cytokine in patients with myasthenia gravis].
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Li Qian-ru, Du Ying, Zhao Hang, Gao Feng, Zhang Qing-yong, Zhang Qin-xian
Аннотация
AIM: To study the relationship between the abnormal differentiation thymocytes and the pathogenesis of myasthenia gravis (MG).
METHODS: The gene expression of some ILs, IFN and their receptors was examined by cDNA array. The percentage of CD45RO(+) and D45RA(+) thymocytes was measured by flow cytometry (FCM). The expression and distribution of CD45RA and CD45RO in the samples of thymus tissues were observed by immunohistochemistry.
RESULTS: The levels of IL-1R, IL-4R, IFNgammaR1, IFNgammaR2, IL-6 and IL-8 in the patients with MG were lower than those of normal controls and there were significant differences between them. But the levels of IL-1, IL-2, IL-4, IL-10, IL-7, IFN-alpha, IFN-beta and IFN-gamma showed no significant changes in patients with MG in comparison with normal controls. The percentage of CD56(+) thymocytes in MG patients 0.56+/-0.33 was significantly lower than that in normal controls(1.78+/-0.69).The CD45RO(+) and CD1a(+) in MG patients increased more significantly those in normal controls. The immunohistochemistry and RT-PCR showed the same result.
CONCLUSION: Aberrant transformation from CD45R0(+)T to CD45RA(+)T has been found in the development of thymocytes of the patients with MG.
METHODS: The gene expression of some ILs, IFN and their receptors was examined by cDNA array. The percentage of CD45RO(+) and D45RA(+) thymocytes was measured by flow cytometry (FCM). The expression and distribution of CD45RA and CD45RO in the samples of thymus tissues were observed by immunohistochemistry.
RESULTS: The levels of IL-1R, IL-4R, IFNgammaR1, IFNgammaR2, IL-6 and IL-8 in the patients with MG were lower than those of normal controls and there were significant differences between them. But the levels of IL-1, IL-2, IL-4, IL-10, IL-7, IFN-alpha, IFN-beta and IFN-gamma showed no significant changes in patients with MG in comparison with normal controls. The percentage of CD56(+) thymocytes in MG patients 0.56+/-0.33 was significantly lower than that in normal controls(1.78+/-0.69).The CD45RO(+) and CD1a(+) in MG patients increased more significantly those in normal controls. The immunohistochemistry and RT-PCR showed the same result.
CONCLUSION: Aberrant transformation from CD45R0(+)T to CD45RA(+)T has been found in the development of thymocytes of the patients with MG.
Предметные рубрики MeSH
AdolescentAdultChildChild, PreschoolCytokinesFemaleFlow CytometryHumansImmunohistochemistryInterferon-alphaInterferon-betaInterferon-gammaInterleukin-1Interleukin-10Interleukin-2Interleukin-4Interleukin-6Interleukin-7Interleukin-8Leukocyte Common AntigensMaleMiddle AgedMyasthenia GravisOligonucleotide Array Sequence AnalysisReceptors, InterferonReceptors, Interleukin-1Receptors, Interleukin-4Reverse Transcriptase Polymerase Chain ReactionThymus GlandYoung AdultInterferon gamma Receptor