Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2024
Analysis of viral diversity in dogs with acute gastroenteritis from Brazilian Amazon.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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de Deus Danielle Rodrigues, Siqueira Jones Anderson Monteiro, Maués Marcelino Antonio Costa, de Fátima Mesquita de Figueiredo Márcia Janete, Júnior Edivaldo Costa Sousa, da Silva Bandeira Renato, da Costa Pinheiro Kenny, Teixeira Dielle Monteiro, da Silva Luciana Damascena, de Fátima Dos Santos Guerra Sylvia, da Silva Soares Luana, Gabbay Yvone Benchimol
Аннотация
Viral gastroenteritis is commonly reported in dogs and involves a great diversity of enteric viruses. In this research, viral diversity was investigated in dogs with diarrhea in Northern Brazil using shotgun metagenomics. Furthermore, the presence of norovirus (NoV) was investigated in 282 stool/rectal swabs of young/adult dogs with or without diarrhea from two public kennels, based on one-step reverse transcription polymerase chain reaction (RT-PCR) for genogroup VI and VII (GVI and GVII) and real-time RT-PCR for GI, GII, and GIV. Thirty-one viral families were identified, including bacteriophages. Phylogenetic analyses showed twelve complete or nearly complete genomes belonging to the species of Protoparvovirus carnivoran1, Mamastrovirus 5, Aichivirus A2, Alphacoronavirus 1, and Chipapillomavirus 1. This is the first description of the intestinal virome of dogs in Northern Brazil and the first detection of canine norovirus GVII in the country. These results are important for helping to understand the viral groups that circulate in the canine population.